JACKIE software/database for gRNA designs is now published at The CRISPR Journal:
https://doi.org/10.1089/crispr.2022.0042
For more information source code, database and genome browser links:
JACKIE page
Press release at ASU FullCircle about this CRISPR Journal paper and our recent Nature Communications paper on genome imaging:
https://fullcircle.asu.edu/research/cultivating-crispr-innovations/