Generate JACKIE.queryDB command

Generate JACKIE.queryDB command to extract all gRNAs filtered for specific properties, within genomic regions, or select best N gRNAs within each region by an ordered list of sort criteria.

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JACKIEdb filename (bigBed format)
Region file (bed format)
Regions (each line e.g., chr1:1-1000 or chr1[tab]1[tab]1000)
Output filename (empty: output to screen [STDOUT])
Select Best
Unselected Sort Criteria [Double click to select] Selected Sort Criteria (Ranked by Priority) [Double click to remove]

Number of sites in cluster to

Size of gRNA cluster to

Percentage GC of Spacer Sequence to

longest run of T to

Total Number of 1,2,3-mismatch sites to

Number of 1-mismatch sites to

Number of 2-mismatch sites to

Number of 3-mismatch sites to