Parent project: [Casilio]

Plasmids available on Genomics-online.com: [Table of Plasmids]

Casilio-ME are DNA methylation editing (ME) modules for Casilio

Project led by Research Scientist, Dr. Aziz Taghbalout.

▲Casilio-ME1 mediated activation of DNA methylation silenced genes.

▲Dual delivery of TET and other proteins involved in DNA demethylation.

▲From left to right: Albert Cheng, Aziz Taghbalout, [Casilio presentation], Menghan Du, Nathaniel Jillette. Photography by Charles Camarda.

Publication:

Taghbalout, A., Du, M., Jillette, N., Rosikiewicz, W. Rath, A., Heinen, C., Li, S., Cheng, A.W. (2019) Enhanced CRISPR-based DNA demethylation by Casilio-ME-mediated RNA-guided coupling of methylcytosine oxidation and DNA repair pathways. Nature Communications 10:4296. doi:10.1038/s41467-019-12339-7  PMID: 31541098 PMCID: PMC6754513

Press release:

An epigenetic erase by Mark Wanner [ http://www.jax.org/news-and-insights/2019/september/an-epigenetic-eraser ]

Grant support:

R01HG009900, P30CA034196, Leukemia Research Foundation New Investigator Grant, CA115783

Plasmids available on Genomics-online.com: [Table of Plasmids]