CREST (Combinatorial RNA Engineering via Scaffold Tagged gRNA) is a new molecular “operating system” that parellels the DNA-Casilio system. CREST allows the simultaneous execution of multiple RNA modulation functions on different RNA targets. It also allows a split architecture for improved RNA editing specificity

Paper: Liu, Z., Jillette, N., Robson, P., Cheng, A.W. (2023) Simultaneous multifunctional transcriptome engineering by CRISPR RNA scaffold. Nucleic Acids Research  51(14): e77, https://doi.org/10.1093/nar/gkad547

Previously, we created CRISPR-based artificial splicing factors  (CASFx) to allow gRNA-programmed activation and repression of alternative exons.

Paper: Du, M.*, Jillette, N.*, Zhu, J.J., Li, S., Cheng, A.W. (2020) CRISPR Artificial Splicing Factors. Nature Communications 11:2973. doi: 1038/s41467-020-16806-4

For plasmids, please visit Addgene page: https://www.addgene.org/browse/article/28196786/