Plasmids available on Genomics-online.com

Plasmids available on Genomics-online.com

pAC# Plasmid Name Link
Intres/Split-Selectable Marker
2-split hygro
pAC10003 pLX-Hygro(1-89)-NpuDnaE(N)-IRES-TagBFP2 7539386
pAC10004 pLX-NpuDnaE(C)-Hygro(90-341)-IRES-mCherry 7539389
pAC10049 pLX-DEST-IRES-Hygro(1-89)-NpuDnaE(N) 7539390
pAC10050 pLX-DEST-IRES-NpuDnaE(C)-Hygro(90-341) 7539391
pAC10051 pLX-[TagBFP2]-IRES-Hygro(1-89)-NpuDnaE(N) 7539392
pAC10052 pLX-[mCherry]-IRES-NpuDnaE(C)-Hygro(90-341) 7539393
3-split hygro
pAC10090 pLX-NpuDnaE(C)-Hygro(90-200)-SspDnaB(N-S0)-IRES-EGFP 7539387
pAC10089 pLX-SspDnaB(C-S0)-Hygro(201-341)-IRES-mCherry 7539388
Other Hygro
pAC10119 pLX-Hygro(1-51)-gp411_N-IRES-TagBFP2 7539394
pAC10120 pLX-gp411_C-Hygro(52-341)-IRES-mCherry 7539395
pAC10135 pLX-Hygro(1-130)-NrdJ1_N-IRES-TagBFP2 7539396
pAC10136 pLX-NrdJ1_C-Hygro(131-341)-IRES-mCherry 7539397
pAC10129 pLX-Hygro(1-258)-IMPDH1_N-IRES-TagBFP2 7539398
pAC10130 pLX-IMPDH1_C-Hygro(259-341)-IRES-mCherry 7539399
6-split hygro internal splits
pAC10213 pLX-gp411_C-Hygro(52-89)-NpuDnaE_N-IRES-GFP 7539400
pAC10214 pLX-NpuDnaE_C-Hygro(90-130)-NrdJ1_N-IRES-GFP 7539401
pAC10215 pLX-NrdJ1_C-Hygro(131-200)-SspDnaB_N-IRES-GFP 7539402
pAC10216 pLX-SspDnaB_C-Hygro(201-258)-IMPDH1_N-IRES-GFP 7539403
2-split neo
pAC10023 pLX-Neo(1-194)-NpuDnaE(N)-IRES-TagBFP2 7539404
pAC10024 pLX-NpuDnaE(C)-Neo(195-267)-IRES-mCherry 7539405
pAC10057 pLX-DEST-IRES-Neo(1-194)-NpuDnaE(N) 7539406
pAC10058 pLX-DEST-IRES-NpuDnaE(C)-Neo(195-267) 7539407
pAC10059 pLX-[TagBFP2]-IRES-Neo(1-194)-NpuDnaE(N) 7539408
pAC10060 pLX-[mCherry]-IRES-NpuDnaE(C)-Neo(195-267) 7539409
2-split puro
pAC10017 pLX-Puro(1-119)-NpuDnaE(N)-IRES-TagBFP2 7539410
pAC10018 pLX-NpuDnaE(C)-Puro(insCys;120-199)-IRES-mCherry 7539411
pAC10053 pLX-DEST-IRES-Puro(1-119)-NpuDnaE(N) 7539412
pAC10054 pLX-DEST-IRES-NpuDnaE(C)-Puro(120-199) 7539413
pAC10055 pLX-[TagBFP2]-IRES-Puro(1-119)-NpuDnaE(N) 7539414
pAC10056 pLX-[mCherry]-IRES-NpuDnaE(C)-Puro(120-199) 7539415
pAC10205 pLX-NLSGFP-IRES-Puro(1-98)-NrdJ1_N 7539416
pAC10206 pLX-iFP4.1-IRES-NrdJ1_C-Hygro(99-199) 7539417
2-split blast
pAC10013 pLX-Blast(1-102)-NpuDnaE(N)-IRES-TagBFP2 7539418
pAC10014 pLX-NpuDnaE(C)-Blast(103-140)-IRES-mCherry 7539419
Casilio
Casilio – Epigenetic Editing and Casilio-ME
pAT1504 pAT1504_pX-SPFE-BbsI(ccdbCam)-5xPBSc 7529437
pAT1503 pAT1503_pX-SPFE-BbsI(ccdbCam)-5xPBSa 7529438
pAT611 pAT611_CMV/CAG_PUFa-hNEIL2-hTET1(CD) 7529439
pAT892 pAT892_CMV/CAG_PUFa-p65HSF1 7529440
pAT635 pAT635_CMV/CAG_hGADD45A-PUFa-hTET1(CD) 7529441
pAT888 pAT888_CMV/CAG_PUFa-hTET1(CD) 7529442
pAC1355 pAC1355-pmax-NLSPUFa_VP64 7529443
pAC1358 pAC1358-pmax-NLSPUFc_VP64 7529444
pAC1411 pmax-3xFLAG_4xNLS_PUFc_2xNLS_p65HSF1 7529445
pAC1451 pmax-BFPKRAB_4xNLS_PUFa_2xNLS_3xFLAG 7529446
pAC1418 pAC1418-pmax-mCBPHAT_4xNLS_PUFa_2xNLS 7529447
pAC1445 pmax-dCas9 7529448
Casilio – Imaging
pAC11001 lenti_dCas9_Blast 7536597
pAC1447 pmax-Clover_PUFc 7536598
pAC11002 pmax-PUF9R_iRFP670 7536599
pAC11003 pmax-PUF9R_mRuby2 7536600
pAC11004 pmax-Clover_PUF48107 7536601
pAC11005 pmax-iRFP670_PUFc 7536602
pAC11006 pCR8-sgRNA-15xPBSc 7536603
pAC11007 pCR8-sgRNA-15xPBS9R 7536604
pAC11008 pCR8-sgRNA-20xPBS48107 7536605
pAC11009 pCR8-sgRNA-20xPBSc 7536606
pAC11010 pCR8-sgRNA-25xPBS9R 7536607
Casilio – Homology Directed Repair
pmax-XRCC3_PUFa 7536609
pmax-BRCA1_PUFa 7536610
pmax-FANCF_PUFa 7536611
pmax-CtIP(T847E)_PUFa 7536617
pAC302 pmax-Cas9 7536612
pmax-CtIP(T847E)_53BP1 7536613
pmax-XRCC3_53BP1 7536614
pmax-BRCA1_53BP1 7536615
pmax-FANCF_53BP1 7536616
pCR8-sgFE-EMX1-5xPBSa 7536618
pCR8-sgFE-CXCR4-5xPBSa 7536619